Abstract

AGUILAR JIMENEZ, Daniel  and  RODRIGUEZ DE LA O, José Luis. Micropropagation and acclimatization of Maguey Pitzometl (Agave marmorata Roezl) in the poblana Mixteca. Rev. colomb. biotecnol [online]. 2018, vol.20, n.2, pp.124-131. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v20n2.77084.

Agave marmorata R. is a plant that adapts to shallow lands and low fertility, it also serves for the retention and conservation of rainwater reducing soil erosion. It is currently found in a wild and scarce form in the Mixteca Poblana (Mexico) where it is intensively used in gastronomy and as a medicinal plant. For this reason, in vitro propagation was proposed as a rescue and conservation strategy from in vitro shoots of Agave marmorata in a basic culture medium of Murashige and Skoog (1 962) 100 %, supplemented with 3 % sugar cane, myo- inositol 100 mg-L-1, agar 0.7 % and 0.40 mg-L-1 thiamine-HCl, where different concentrations of 6-Benzyl-adenine (BA) and indole-3-acetic acid (IAA) were added separately by a 5x5 factorial arrangement. The number and length of shoots and roots obtained in vitro were evaluated. For the plants in acclimation only the percentage of survival in different substrates was evaluated. Los datos de las variables se sometieron a un análisis de varianza y a una prueba de medias de Tukey (p=0.05). The best response for shoot length, number and length of roots was in the MS 100 % medium adding 10 mg-L-1 of IAA and the proliferation of new shoots was promoted with the addition of BA and IAA in the same concentration. Finally, 100 % survival of the seedlings was obtained in the substrates of peat moss plus agrolita and peat moss plus river sand.

Keywords : in vitro; organogenesis; propagation; substrate.

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