Resumo

RAMIREZ BABATIVA, Daniel Ferley. Adaptation of the acreman method for cleaning plants in lemna minuta (araceae: lemnoideae). Rev. colomb. biotecnol [online]. 2019, vol.21, n.1, pp.128-134. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v21n1.53219.

Lemna minuta is a floating macrophyte widely distributed in lentic ecosystems, which may be useful for development of ecophysiological and ecotoxicological experiments, due to its sensitivity to water pollutants such as toxins and heavy metals. Axenic cultures and cleaning techniques of fronds from their populations under laboratory conditions have not yet been defined. Acreman method was modified in exposure time and concentration of sodium hypochlorite for disinfection from native colonies of species belong to genus Lemna L. (Lemnoideae). The colonies were obtained from an urban wetland of Bogota city, and they were acclimatized and disinfected at different times with hypochlorite solutions. The most suitable results for the removal of epiphytic algae and other microorganisms of the fronds, without showing high mortality of colonies, were obtained, respectively, in 0,5 % (45 and 30 seconds) and 0,25 % (60 seconds) hypochlorite concentrations. By contrast, the exposure time of 60 seconds proposed by Acreman method (0,5 %) resulted in total mortality of fronds (100 %). This report suggests use a solution of 0,5 % sodium hypochlorite in an exposure time of 45 seconds or less for disinfect colonies of L. minuta with experimental purposes.

Palavras-chave : contamination; frond; hypochlorite; Lemna minuta; culture media.

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