Resumo

BERTEL MESA, Lina Marcela; BETANCUR HURTADO, Cesar Augusto  e  OVIEDO ZUMAQUE, Luis Eliécer. Identification of Bacillus toyonensis in faeces of zebu cattle in the Sucre Department, Colombia. Rev. colomb. biotecnol [online]. 2019, vol.21, n.2, pp.12-21.  Epub 08-Jan-2020. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v21n2.69421.

Probiotic characteristics of isolated native strains from zebu cattle were evaluated and molecular identification of one of these strains was performed to consider their subsequent application as microbial additives for bovine feeding. Manure samples were collected from growing zebu calves. Bacteria and yeasts were isolated and the probiotic capacity of these strains was determined by means of tests such as; resistance to bile salts (0.05, 0.1, 0.15 and 0.3 %), resistance to acid pH (pH 3, 4, 5.6, 7) tolerance to NaCl (2, 4, 6, 8, 10 %), antagonistic activity (Salmonella sp. and Escherichia coli), gas production from glucose, and growth at different temperatures (37 and 40 °C). Preliminary identification of the strains was performed by techniques such as: Gram staining, endospore staining (Wirtz method), catalase and oxidase test. One of the strains that exceeded the probiotic probes was selected for its identification employing molecular methods and the phylogenetic analysis was performed using the NCBI Reference Sequence (RefSeq) database. The trials were organized in a Completely Randomized Design, whose results obtained in triplicate were systematized in the Statgraphics Centurion XVI software and the effect of the different treatments was analyzed statistically through an analysis of variance. 10 native strains were isolated, identifying Bacillus toyonensi molecularly. This research represents the first molecular report of B. toyonensis in manure from growing zebu calves in the Sucre Department, which has been widely used as a probiotic in animal nutrition.

Palavras-chave : bovine; strains; probiotic; calves.

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