Abstract

TORRES PEREZ, Maria Paulina; VITOLA ROMERO, Deimer  and  PEREZ CORDERO, Alexander. Bioremediation of mercury and nickel by endophytic bacteria of aquatic macrophytes. Rev. colomb. biotecnol [online]. 2019, vol.21, n.2, pp.36-44.  Epub Jan 10, 2020. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v21n2.79975.

Environmental pollution has been categorized as one of the main problems that affects the health of different life forms. Endophytic bacteria (BE) are able to improve the nutritional status of plants and remove soil contaminants. The Mojana subregion works as a river depreciation zone. In this study, samples of aquatic macrophytes were collected from the marshes of Ayapel, San Marcos and San Benito Abad, from which endophytic bacteria were isolated. The population densities of these bacteria and their respective tolerance to nickel and mercury heavy metals were quantified. Subsequently, the tolerant strains were molecularly identified and their ability to promote plant growth was evaluated, from which the population densities of endophytic bacteria and tolerance to heavy metals were isolated and determined. , to the tolerant isolates and capable of growing in nickel and mercury, plant growth promotion was evaluated, finally they were identified at the molecular level. A total of 182 morphotypes of endophytic bacteria were isolated, the highest population density averages were obtained in the macrophytes of the swamp of San Benito Abad located at the coordinates coordinates 8 ° 55'32.81'' N and 75 ° 1 '13.72 "W , lo. The highest averages of mercury and nickel tolerant morphotypes were found in the Ayapel swamp at coordinates 8 ° 18'51.10'' N and 75 ° 8'8.26 "O. L The results show that the isolates with the greatest biotechnological potential are BAT6, BAR2 and PAT2, where the first two have a 100% homology with the species Lysinibacillus fusifomis and the genus Enterobacter, respectively, while the third isolation had a homology of 96% with the species Burkholderia cepacia show that the isolated BAT6, presented 100% homology with the species of bacteria Lysinibacillus fusifomis; BAR2 100% homology with the bacterial species belonging to the genus Enterobacter and PAT2 96% homology with the species Burkholderia cepacia. The present study reports for the first time the presence of Lysinibacillus fusifomis and Burkholderia cepacia associated with macrophytes in swampy bodies of Sucre and Córdoba.

Keywords : contamination; heavy metals; phytoremediation; plants.

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