Resumen

ROJAS-BEDOYA, Laura; GOMEZ-LOPEZ, Catherine  y  MARIN-PAREJA, Nathalia. Extraction of metabolites from Calendula officinalis and evaluation of their colorant and antibacterial capacity. Rev. colomb. biotecnol [online]. 2020, vol.22, n.1, pp.60-69. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v22n1.79999.

In this work, different bioactive compounds were obtained by different extractions from Calendula officinalis. The effect of different extraction time and temperature on the quantity of bioactive compounds was investigated. The extracts were quantified by UV-visible spectrometric analysis. The effect of extraction technique on both the colorant power and antibacterial capacity of metabolites obtained was evaluated. Colorant power was evaluated by UV-visible spectrometric and dyeing ability in dairy product. Antibacterial activity of extracts was evaluated using Escherichia coli and Salmonella typhimurium by diffusion technique in well. Outstanding extraction results were obtained by ultrasound extraction technique performed during 2 hours, at 30 °C and ethanol 96% as solvent. The same treatment showed the best result in colorant power and dyeing ability. The extracts obtained by ultrasound had a slightly inhibitory effect of growth on E. coli in comparison with the other techniques. None of the extracts obtained from the different techniques reported a significant inhibition on the growth of S. typhimurium. Therefore, ultrasound-assisted extraction is considered as an alternative process for obtaining the bioactive compounds from C. officinalis with high concentration, colorant power and antibacterial activity.

Palabras clave : metabolites; dyeing ability; antimicrobial activity; natural additive; common marigold.

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