Abstract

CHAMORRO-ANAYA, Lina María; CHAMORRO-ANAYA, Leonardo Miguel  and  PEREZ CORDERO, Alexander. Bacillus cereus endophytic bacterial plant growth promoter. Rev. colomb. biotecnol [online]. 2020, vol.22, n.2, pp.18-23.  Epub Feb 15, 2021. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v22n2.81723.

The Lippia origanoides plant has been widely studied due to the antimicrobial and antifungal effect of its extracts and essential oils, which have been tested against a large number of pathogenic microorganisms. However, there is little literature that records the diversity of endophytic bacteria associated with this plant species. The objective of the work was to evaluate in vitro the plant growth promotion capacity of endophytic Lippia origanoides bacteria in the municipality of Sincelejo-Sucre, Colombia. In this study, endophytic bacteria were isolated in R2A agar culture medium from different tissues, population density (CFU / g of tissue) was evaluated by surface counting and the promotion of plant growth qualitatively in specific selective media. Significant differences were observed for the population density of endophytic bacteria regarding tissue type, with higher values in the root (2.0 x 1010 / g root), followed by the stem (1.3 x 1010 / g stem) and leaves (9.2 x 109 / g sheet). A total of 20 endophytic bacteria were obtained, which two showed phosphate solubilizing capacity, biological nitrogen fixation, production of siderophores and ACC deaminase. The TLO5 and RLO4 morphotypes were molecularly identified as Bacillus cereus, showing good results in promoting plant growth.

Keywords : antimicrobial; population density; Lippia origanoides; microorganism; Plant tissue, Bacillus cereus.

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