Abstract

CORONADO, Núñez; GONZALEZ ROSAS, Héctor  and  FERNANDEZ PAVIA, Yolanda L.. Micropropagation of Calibanus hookeri (lem.) trel (1911). A threatened species. Rev. colomb. biotecnol [online]. 2021, vol.23, n.1, pp.46-54.  Epub Aug 11, 2021. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v23n1.80873.

The species Calibanus hookerii belonging to the family Aspagaceae is registered in the NOM-059-SEMARNAT-2010 cataloged in danger of extinction and therefore is necessary of propagation methods that assure its conservation and its propagation. In vitro propagation is a viable alternative for endangered plant species. The present investigation reports the protocol for the micropropagation of Calibanus hookerii. This was achieved by seed germination without test in MS medium supplemented with 2.5, 5.0 and 7.0 mg L-1 of 6-benzyladenine (BA), kinetin (K), 2-isopentyl-adenine (2iP) and tidiazuron (TDZ). The variables to be measured were percentage of germination, number and length of shoots produced by seed. The most efficient treatment was 5 mg L-1 of BA producing an average of 26 shoots per seed, the worst treatment was with 2.5 K only produced 2 shoots per seed, of the four cytokinins used 2ip treatment in only one study of the Performed showed results (7 mg L-1) producing 6 shoots per seed. Regarding the length of the shoot, no treatment exceeded the control (8.07cm). Finally, in vitro germination was high (56-97%) in all treatments.

Keywords : direct organogenesis; in vitro culture; cytokinins; rescate.

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