Resumo

MONTOYA BARRETO, Sandra; RESTREPO FRANCO, Gloria María  e  CALVO RODRIGUEZ, Claudia Lorena. Ligninolytic activities and production of Ganoderma lucidum basidiomes in solid culture supplemented with manganese. Rev. colomb. biotecnol [online]. 2022, vol.24, n.2, pp.46-58.  Epub 07-Mar-2023. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v24n2.95288.

Ganoderma lucidum is a white rot basidiomycete specially studied for its medicinal attributes. However, the information on the supplementation of the substrate with metals such as Zn, Li, Mn, Cu and others is scarce. Even knowing that the presence of metals in the substrates improves the characteristics of the basidiomes produced and increases their productivity. The objective was to evaluate the enzymatic activities laccase (Lc) and manganese peroxidase (MnP). The fructification and biological efficiency (BE) of G. lucidum in solid culture formulated with agroindustrial residues, (oak sawdust, coffee husk, bran corn) supplemented with two levels of manganese II salt, (0.05 % and 0.1 % w/ w) and a formulation without addition of manganese II salt. Enzymatic activities were determined during 98 days of the production cycle, with weekly sampling. Treatment one (0.05 % w/w MnSO4.H2O) provided the highest BE of the culture with 25.90 ± 0.54% and the highest titers of ligninolytic activities, in the time with 0.7299 EU/g d.s for MnP at 35 days of fermentation and 4.1760 EU/g d.s for Lc activity at 42 days of process, in relation to treatments two and control. Likewise, there was a decrease in the fungus culture cycle for treatments one (83 days) and two (95 days) compared to the control treatment (117 days). The results of this work are promising for industrial growers of G. lucidum, since the supplementation of the substrates with Mn increase the productivity of the cultures.

Palavras-chave : biological efficiency; ligninases; fructification; substrates supplemented with manganese sulfate II.

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