Resumo

MANCERA-MARTINEZ, Diego N.; CHEGWIN ANGARITA, Carolina  e  SERRATO BERMUDEZ, Juan Carlos. Biomass production and chemical characterization of Lentinula edodes grown in a bioreactor under different conditions. Rev. colomb. biotecnol [online]. 2023, vol.25, n.1, pp.46-56.  Epub 11-Dez-2023. ISSN 0123-3475.  https://doi.org/10.15446/rev.colomb.biote.v25n1.96477.

Biochemical engineering plays an important role in the technological development of the processes for obtaining either mushroom fruiting bodies with medicinal potential for direct consumption, or in the manufacture of nutraceuticals and/or dietary supplements. This research implemented a methodology in a bioreactor for the cultivation of the Lentinula edodes macromycete fungus, commonly known as Shiitake, with the aim of evaluating the effect of agitation speed and air flow on the production of biomass and metabolites of median and low polarity. Thus, it was found that the highest yield and productivity of biomass production is achieved at conditions of 80 rpm and 0,50 vvm, obtaining 0,0260 g/g and 0,00433 g/L*h respectively. Likewise, in the study of the content of metabolites it was concluded that for the protein 33,4% of the lyophilized mycelium was reached at conditions of 140 rpm and 0,75 vvm, the sterols reached a percentage of 89,32% of the extract. in DCM at 80 rpm and 0,5 vvm. The results of the GC-MS analysis confirm the wide variety of compounds that can be obtained from a bioreactor culture of the Lentinula edodes fungus.

Palavras-chave : Macromycete; sterols; sugars; protein; biotechnology.

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