Abstract

RODRIGUEZ, Yeray et al. PHYSICOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS COLLECTED IN THE ATLÁNTIC DEPARTMENT, COLOMBIA. rev.udcaactual.divulg.cient. [online]. 2012, vol.15, n.2, pp.303-311. ISSN 0123-4226.

A comparison of the physicochemical properties of the propolis collected in the Galapa, Sabanalarga, Santo Tomás and Juan de Acosta's municipalities of the Atlantic department (Colombia) was made. Additionally, the in vitros antioxidant activity of ethanol extracts of propolis (EEP) through the DPPH, ABTS, FRAP and ORAC methods was established. The physicochemical analysis of propolis indicated values between 8.48 - 24.21%, 1.77 - 6.07%, 55.57 - 77.93%, 5.74 - 11.69% and 0.35 - 3.86%, for EEP, waxes, insoluble residues, humidity and ashes respectively. The determination for the total phenolic content was between 63.72 and 94.55mg of gallic acid/g of sample. The EEP's specific absorbance ranged from 26 to 96 and the total flavonoid content showed values between 1.90 and 3.36mg of quercetin/g of sample. The extract that showed the highest antioxidant capacity was the propolis collected in the municipality of Galapa with the following values, DPPH: 190,41 TEAC (µmol/g), ABTS: 1918,41 TEAC (µmol/g), FRAP: 321,27 AEAC (µmol/g), and the second highest value in ORAC (1964,80 µmol TROLOX/g of propolis). The results obtained indicated that the propolis studied showed a variation in its physicochemical properties. Some of the estimated physicochemical parameter values did not fulfill the established international quality regulations. Nevertheless, the ethanol extracts of the evaluated propolis presented a high content of phenols, related with the antioxidant properties found experimentally.

Keywords : Propolis; phenolic compounds; physicochemical properties; antioxidant properties.

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