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vol.15 issue2PHYSICOCHEMICAL CHARACTERIZATION AND EVALUATION OF ANTIOXIDANT ACTIVITY OF PROPOLIS COLLECTED IN THE ATLÁNTIC DEPARTMENT, COLOMBIAMOLECULAR CHARACTERIZATION OF YAM (Dioscorea alata L.) ACCESSIONS FROM THE COLOMBIAN CARIBBEAN REGION author indexsubject indexarticles search
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Revista U.D.C.A Actualidad & Divulgación Científica

Print version ISSN 0123-4226

Abstract

CRUZ B., Mónica  and  BELLO, Felio J.. PRIMARY CELL CULTURES DERIVED FROM Sarconesiopsis magellanica (LE GUILLOU, 1842) (DIPTERA: CALLIPHORIDAE). rev.udcaactual.divulg.cient. [online]. 2012, vol.15, n.2, pp.313-321. ISSN 0123-4226.

The present work was aimed at obtaining and characterizing cell cultures from Sarconesiopsis magellanica embryonic tissue (Diptera: Calliphoridae). This fly is important by its applications in the establishment of post-mortem interval. This study evaluated seven culture medium (Grace, Grace/L15, MM, VP12, MM/VP12, Eagle and Schneider), supplemented with 20% fetal bovine serum. The adhesion, cell growth and proliferation were observed in L15 medium, Schneider and Grace/L15, achieving better results in the last two. The confluent monolayer was present in an average of twelve days after that the explants were made. The growth pattern of the primary cultures showed the presence of vesicles with cells adhering to its walls, which helped to form a confluent monolayer. The predominant cell morphology in monolayer correspond to fibroblastoid a lesser extent to epithelioid, showing the first similar to nerve cells. Additionally, there was in the early days of primary cultures, especially in some clusters of cells, contractile movement, similar to those made by muscle cells. These primary cell cultures derived from S. magellanica represent potentially suitable substrates for further testing of susceptibility to infection with arboviruses and parasites.

Keywords : Flies; culture media; embryonic tissue; vesicles; cell morphology.

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