Abstract

DURAN, Marlene; MONTERO, Piedad  and  MARRUGO, Yesid. METHANOLIC EXTRACTS OF GUAVA (Psidium guajava L.) AND MANGO CRUSTS (Mangifera indica L.): CITOTOXIC, ANTIHEMOLITIC EFFECT AND IN THE MORPHOLOGY OF ERYTHROCYTES MEMBRANE. rev.udcaactual.divulg.cient. [online]. 2013, vol.16, n.2, pp.327-334. ISSN 0123-4226.

In the Caribbean coast green fruits are consumed at high frequency and in this process the traders of these products remove the crusts as waste products, which could be exploited in the food industry and in environmental improvement. The objective of this study was to determine alterations in biological membranes using as a model the erythrocyte, the antihemolytic activity induced by hydrogen peroxide, and the membrane deformability caused by different concentrations (200, 100, 50 and 20ug/mL) of the total methanol extracts of crusts of the tested fruits. The cytotoxicity was performed using the trypan blue exclusion in mononuclear of peripheral blood. Antihemolytic activity was determined by spectrophotometry at a wavelength of 520nm, the deformability was made by counting erythrocytes morphologically altered specifically equinocyte forms. Extracts of mango and guava crusts were not cytotoxic at the tested concentrations; they showed cell viability higher than 80%. They inhibited the hemolysis in the presence of H₂O₂, the guava extract at 100μg/mL recorded the greatest inhibition of induced hemolysis. Higher percentages of morphological changes were observed when erythrocytes were exposed to concentrations of 100 and 200μg/mL. In extract of guava crust the concentration was 200μg/mL, it showed 47% of echinocyte, whereas mango extract 200μg/ mL showed 90% of echinocyte.

Keywords : Erythrocyte membrane; extracts; oxidative stress; cytotoxicity.

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