Resumo

GIRALDO PINZON, Etna; PEREZ CARDENAS, Jorge; AGUILAR MARIN, Sandra  e  LINARES VILLALBA, Sergio. PREVALENCE OF BOVINE FASCIOLOSIS IN A ZONE OF CALDAS COLOMBIA WITH EVIDENCES OF THE DISEASE. rev.udcaactual.divulg.cient. [online]. 2016, vol.19, n.1, pp.139-148. ISSN 0123-4226.

Fasciolosis is a parasitic disease that affects ruminants, horses, pigs and other species; it is the cause of significant economic losses and can also parasitize humans. In this research, the frequency of the disease using three detection methods in twenty-six farms, located in the municipalities most prevalent according to reports from the Slaughtering Central of Manizales-Caldas in the department of Caldas was evaluated. The coproscopic, PCR and ELISA methods in feces and serum of randomly selected cattle farms with a system of dairy production, located in the Colombian Andes, between 1980 3721m, were used. 414 stool samples were obtained and processed with the Dennis modified technique. DNA was extracted from stool using a commercial kit and was amplified with specific primers for standard PCR. In serum samples, the presence of immunoglobulin G (IgG) against Fasciola hepatica was detected through the use of a specific commercial kit for this purpose. The study area was divided into four zones for the study purposes. The prevalence of fascioliasis by the coproscopic test was 12.3%, by ELISA of 19.1%, and 67.2% by PCR. Zone three displayed the highest proportion of positive animals by the three methods, with high odds ratio and high statistical significance; lymnaeid snails were found in aquatic environments suitable for development, allowing perpetuate the inoculum. The presence of bovine fasciolosis in the studied region was confirmed; additional studies to validate PCR as a method by the fasciolosis detection are required.

Palavras-chave : PCR; Fasciola hepatica; snails; zoonoses; Elisa; coprology.

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