Abstract

GONZALEZ TORRIVILLA, César; HERNANDEZ, Lorena  and  VAILLANT BARKA, Fabrice. ANALYSIS OF JUICE BLACKBERRY (Rubus adenotrichos) SWEETENED WITH STEVIA (Stevia rebaudina Bertoni), A METABOLOMICS APPROACH. rev.udcaactual.divulg.cient. [online]. 2017, vol.20, n.1, pp.121-129. ISSN 0123-4226.

The purpose of the research was to analyze, through a metabolomic study, the overall composition of a microfiltration blackberry juice (Rubus adenotrichous) sweetened with dried leaves of stevia (Stevia rebaudiana Bertoni) and its comparison with analogues obtained by conventional heat treatment, sweetened with sucrose. The spectral information was obtained by an undirected approach. all possible metabolites produced in the four samples (unpasteurized and pasteurized microfiltered juice, sweetened with stevia and sucrose) were measured simultaneously. Analyses were conducted in series using a Waters Acquity Ultra Performance Liquid Chromatography system (UPLC) equipped with a photodiode array detector (PDA) and coupled with Electrospray Ionization and Quadrupole Time-Of-Flight Mass Spectrometry (ESI-Q-TOF/MS. SPL format files, provided by the UPLC, in the analysis of the samples were converted to NetCDF format, the respective analyzes were performed by the MZmine software. all ions were detected, discriminating presence or not in the different samples. The mass (molecular) and the retention time were recorded by the mass spectrometer. The results of the statistical analysis applied confirmed the variation in composition microfiltration blackberry juice sweetened with sucrose and stevia when subjected to heat treatment step, corroborating statistically significant differences between them, which allows to affirm that the treatment for the formulations studied generates a variation of the ions present.

Keywords : Ions; metabolites; microfiltration; pasteurization(CAB Thesaurus).

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