SciELO - Scientific Electronic Library Online

 
vol.20 issue2STIMULATION OF GERMINATION OF SPORES AND ROOT COLONIZATION OF Diversispora trimulares BY FLAVONOIDS IN Nicotiana glauca ROOT EXUDATESINTEGRATED DISEASE MANAGEMENT OF FUSARIUM WILT OF TOMATO [Fusarium oxysporum f. sp. lycopersici (SACC.) W.C. SNYDER & H.N. HANSEN]:: A REVIEW author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Revista U.D.C.A Actualidad & Divulgación Científica

Print version ISSN 0123-4226

Abstract

ARIAS, Juan Pablo et al. CARDIAC GLYCOSIDES, PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY FROM PLANT CELL SUSPENSION CULTURES OF Thevetia peruviana. rev.udcaactual.divulg.cient. [online]. 2017, vol.20, n.2, pp.353-362. ISSN 0123-4226.

Cell suspension cultures of Thevetia peruviana were established under dark for 19 days to investigate kinetic behavior related to biomass, substrate, cardiac glycoside, polyphenols, reactive oxygen species and anti-oxidant activity. The results showed high biomass production (18.80gDW/L) as well as sucrose consumption in 7 days. Preferential glucose over fructose consumption was observed. Intracellular production of cardiac glycosides reached 2.58mg DE/gDW at day 19. Highest extracellular production was reached between day 2 and 7 (6.19mg DE/L). Highest extracellular phenolic content was 80.61 ± 5.16mg GAE/L at day 7. Intracellular phenolic content increased to 2.76 ± 0.14mg GAE/gFW at day 7 and remained constant until day 19. ROS production at day 7 could be related to sucrose and glucose total consumption. Pearson Product-Moment Correlation Coefficient (ρ) showed that the phenolic compounds in cell suspension cultures of T. peruviana were responsible for the observed anti-oxidant activity. All together, these results give the first steps in metabolic behavior in cell suspension cultures of T. peruviana.

Keywords : Plant metabolites; Anti-oxidant activity; Reactive Oxygen Species; Phenols; Plant cell suspension culture; Thevetia peruviana.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License