Resumen

SALAZAR GONZALEZ, Claudia et al. Characterization of Fusarium spp. associated with the basal rot of the green onion. rev.udcaactual.divulg.cient. [online]. 2020, vol.23, n.1, e1471.  Epub 30-Jun-2020. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v23.n1.2020.1471.

The department of Nariño occupies the fourth place as producer of green onion nationwide. In recent years, its production and cultivation area has been reduced by multiple limitations, highlighting as the most important, the susceptibility to the attack of fungi causing radical problems, which harm the productive system and profitability. This study was carried out in order to morphologically and molecularly characterizes Fusarium populations associated with green onion basal rot disease. For this, in Pasto, Potosi and Buesaco municipalities, plants were collected with basal rot symptoms, tissue deterioration and root necrosis. In the Plant Health laboratory of the University of Nariño, tissues were planted in PDA medium and subsequently the isolates were purified for further morphological and molecular characterization. The morphological study was carried out using taxonomic keys for the genus Fusarium and molecular characterization with specific primers for the genus Fusarium, and by sequencing. Finally, a phylogenetic analysis of the intraspecific variability was carried out. Morphological and molecular characterization results corroborate the presence of two species within the genus associated with this pathology, F. oxusporum f sp. cepae and F. solani. Phylogenetic analyzes show high intraspecific variability between the isolates of F. oxysporum and F. solani, forming two Fusarium oxysporum (FOSC) complexes and Fusarium solani (FSSC), evidencing that these species do not start from a common ancestor.

Palabras clave : Japanese bunching onions; Fusarium spp.; fungal morphology; internal transcribed spacers; elongation factor 1-α; phylogeny.

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