Abstract

SIMANCAS-ESCORCIA, Víctor  and  DIAZ-CABALLERO, Antonio. Effect of the absence of fetal bovine serum on the morphology and organelles of fibroblast cells. rev.udcaactual.divulg.cient. [online]. 2020, vol.23, n.2, e993.  Epub July 30, 2020. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v23.n2.2020.993.

Fibroblasts are constituent cells of connective tissues. Gingival fibroblasts (GFs), cells responsible for the synthesis of the extracellular matrix (ECM) in the gingival connective tissue, participate in the regulation of healing and repair processes of the gingiva. Due to their regenerative potential, GFs could be cells capable of contributing to improve the healing processes at the local and systemic level and even be used as a useful cellular model in understanding of the physiopathological aspects of the oral cavity. The aim of the present work was to describe the impact of the concentration of fetal bovine serum (FBS) on the survival, growth and expression of cell markers in GFs. Cell cultures of GFs were performed for 7 days using DMEM culture medium (Dulbecco's Modified Eagle's medium) in the absence and presence of 10% FBS. Morphological and immunohistochemistry analyzes of actin, mitochondria, lysosomes and endoplasmic reticulum (ER) were used to evaluate the impact of FBS concentration on GFs. The results indicate that GFs cultured in the presence of 10% FBS had a higher growth rate compared to GFs cultured in the absence of FBS. The marking of the cellular elements indicates the absence of alterations in the cellular organelles of the GFs when they are cultured in the absence of FBS. In conclusion, GFs are capable to surviving, proliferating and conserving their morphological characteristics when they are cultured in the presence and absence of FBS.

Keywords : Culture media; Cell proliferation; Mitochondria; Lysosomes; Endoplasmic reticulum.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )