Abstract

FAJARDO-ARGOTI, Catalina; JURADO-GAMEZ, Henry  and  PARRA-SUESCUN, Jaime. Viability of microencapsulated Lactobacillus plantarum under simulated gastrointestinal conditions and inhibition against Escherichia coli O157:H7. rev.udcaactual.divulg.cient. [online]. 2021, vol.24, n.1, e1733.  Epub Apr 21, 2021. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v24.n1.2021.1733.

Lactobacillus inhibit multiple pathogens that cause food poisoning, and their mechanism of action is related to the modulation of the immune system. E. coli O157:H7 is considered a causal microorganism of alterations, mainly at intestinal and renal level, and is generally found in contaminated or spoiled food matrices. The incidence of this type of problem has been related to antibiotic resistance, which limits its effective control and mitigation. Therefore, the objective of this work was to evaluate the inhibitory effect of microencapsulated Lactobacillus plantarum in vitro on Escherichia coli O157:H7, in order to find innocuous strategies for the control of pathogens. Fermentation kinetics were determined by evaluating variables such as sugar consumption, protein production, acidity, pH and logarithmic phase (CFU/mL). Microencapsulation was performed by spray drying, using inulin and maltodextrin as encapsulating materials. The viability of L. plantarum was determined under simulated gastrointestinal conditions. In addition, the physical characteristics of the microencapsulated microorganism and the inhibitory effect of L. plantarum and its supernatant on E. coli O157:H7 were evaluated. Subsequently, the susceptibility of both strains to different antibiotics was evaluated. As a result, resistance of both strains to some of the antibiotics evaluated, such as penicillin, was found. The lactic strain and the supernatant inhibited the growth of E. coli O157:H7. L. plantarum showed optimal viability at simulated gastrointestinal conditions after 45 days of storage (1.4x107-3.0x1010CFU/150µL). Microencapsulation increases its viability and establishment in the host.

Keywords : Animal health; Probiotic; Lactobacillus; Pathogen.

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