Resumo

GARCIA-TORRES, Andrea et al. Prevalence of RNA viruses in certified seed-tubers of Solanum tuberosum cv. Diacol Capiro from the highlands of Cundinamarca and Boyacá. rev.udcaactual.divulg.cient. [online]. 2022, vol.25, n.2, e2151.  Epub 15-Dez-2022. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v25.n2.2022.2151.

Diacol-Capiro is one of the most important potato (Solanum tuberosum) cultivars in Colombia with most production concentrated in the provinces of Cundinamarca and Boyacá. Unfortunately, this crop is seriously affected by several viruses that compromise the quality of tubers and yields. In this work, it was evaluated the prevalence of the RNA viruses: PLRV, PVY, PVX, PVS, PVV, PYVV, PMTV, and PVB in certified tuber-seed sprouts produced in the highlands of Cundinamarca and Boyacá by RT-qPCR. Results revealed a prevalence of 100 % for PVS, PVX, and PYVV; 75 % for PLRV, 50 % for PVY, 37.5 % for PMTV, and 12.5 % for PVB. Additionally, high-throughput sequencing from a sprout´s bulk sample was used to gather genomic information of infecting viruses, which resulted in a partial PVY sequence, and a complete PLRV genome. Phylogenetic analysis revealed that both assemblies cluster within clades comprising other Colombian isolates with more than 97 % nucleotide sequence identity. These findings highlight the need to update potato seed-tuber certification programs in Colombia with the implementation of more sensitive molecular tests.

Palavras-chave : High-throughput sequencing; Plant viruses; Potato seed; RT-qPCR; Virus detection.

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