Abstract

MAYA, Andrés F.; DURANGO, Yesica; GOMEZ-PINEREZ, Luz M.  and  GOMEZ, Giovan F.. Improvement of a non-commercial method for DNA extraction from flies of medico-legal interest. rev.udcaactual.divulg.cient. [online]. 2023, vol.26, n.1, e1946.  Epub Feb 22, 2023. ISSN 0123-4226.  https://doi.org/10.31910/rudca.v25.n2.2022.1946.

Obtaining DNA from flies of medico-legal interest is relevant for a variety of applications. Although commercial extraction methods offer optimal DNA, their routine use is limited in some settings. In this context, the use of non-commercial methods constitutes an alternative in laboratories with limited resources however, its optimization is key to improving the workflow and the results. This work evaluated the impact of variations to a saline precipitation method on the concentration and purity of the recovered DNA. No significant differences were found in the concentration of extracted DNA between the different incubation times tested during the extraction phase. In contrast, the increased volume of absolute ethanol in the DNA precipitation phase significantly improved the concentration of DNA obtained. The proposed modifications reduce the runtime and DNA concentration obtained compared with the original protocol.

Keywords : Forensic entomology; Nucleic acids; Arthropods; DNA extraction; Optimization.

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