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Revista Colombiana de Cancerología

versión impresa ISSN 0123-9015

Resumen

BARRERA-HERRERA, Luis Eduardo; ABELLO, Yubelly; RUIZ, Nathalie  y  RODRIGUEZ-URREGO, Paula Andrea. Comparison between cytocentrifugation techniques and liquid-based SurePathTM for the processing of non-gynaecological specimens. rev.colomb.cancerol. [online]. 2016, vol.20, n.1, pp.10-16. ISSN 0123-9015.  https://doi.org/10.1016/j.rccan.2016.01.001.

Objective: The objective of this study was to evaluate the routine use of liquid based cytology for non-gynecologic specimens in our laboratory, comparing morphological characteristics and diagnostic agreement between the two techniques: conventional cytology cytospin, and SurePath liquid-based cytology. Study design: A total of 109 consecutive non-gynecologic specimens were processed, split into two, and then prepared using the two techniques. All slides were reviewed by the same cytopathologist, who evaluated: cellularity, preservation, obscuring elements (inflammation, bleeding, mucus, etc.), and presence of diagnostic groups for each final diagnostic category. When available, an evaluation of the biopsy was performed retrospectively. Results: Good agreement was observed by diagnostic category in 84% of specimens, with a good Kappa index (0.65). The proportion for each category: negative, atypical, and positive was 69%, 18%, and 11%, respectively, for cases processed by liquid-based cytology and 83%, 7%, and 9%, respectively, for conventional cytology cytospin. Conclusion: Liquid based cytology is equivalent to conventional cytology cytospin when cases are grouped by category (good agreement, kappa index 0.651), and is superior in quality because the specimen is well preserved, has a clean background and fewer obscuring elements allowing a better morphological examination.

Palabras clave : Cytology techniques; Validation studies; Non-gynaecological specimens.

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