Abstract

CADAVID GUTIERREZ, Luis Fernando; PEREZ CASTILLO, José Nelson; ROJAS QUINTERO, Cristian Alejandro  and  VERA PARRA, Nelson Enrique. Automation of functional annotation of genomes and transcriptomes. Tecnura [online]. 2014, vol.18, n.spe, pp.90-96. ISSN 0123-921X.  https://doi.org/10.14483/udistrital.jour.tecnura.2014.DSE1.a08.

Functional annotation represents a way to investigate and classify genes and transcripts according to their function within a given organism. This paper presents Massive Automatic Functional Annotation (MAFA - Web), which is an online free bioinformatics tool that allows automation, unification and optimization of functional annotation processes when dealing with large volumes of sequences. MAFA includes tools for categorization and statistical analysis of associations between sequences. We have evaluated the performance of MAFA with a set of data taken from Diploria-Strigosa transcriptome (using an 8-core computer, namely E7450 @ 2,40GHZ with 256GB RAM), processing rates of 2,7 seconds per sequence (using Uniprot database) and 50,0 seconds per sequence (using Non-redundant from NCBI database) were found together with particular RAM usage patterns that depend on the database being processed (1GB for Uniprot database and 9GB for Non-redundant database). Aviability: https://github.com/BioinfUD/MAFA.

Keywords : Annotator; Functional annotation; Gene ontology; High Throughput Sequencing.

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