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Infectio

Print version ISSN 0123-9392

Abstract

PINZON, JANNETH ORQUÍDEA et al. Molecular characterization of Acinetobacter baumannii isolations from a burns unit in a third level attention hospital in Bogotá. Infect. [online]. 2006, vol.10, n.2, pp.71-78. ISSN 0123-9392.

Objective.This study is a fenotypical and genetical description of Acinetobacter baumannii carried out in a ten month follow-up at the Unidad de Quemados from the Hospital Simon Bolívar in Bogotá. Materials and methods. 25 patient and 3 environmental isolates were typified by REP-PCR. Hodge’s test and double disc sinergy were done for microbiologic carbapenemase detection. Isoelectric points were determined by betalactamases isoelectric focusing. Gene bla OXA23, bla OXA24, bla VIM, bla IMP detection was carried out through PCR. Results. Isolate genotypification allowed their classification in nine groups based on similarities greater than 0.85. The E group consisted of 39.2% of the isolates, a clone formed by three environmental isolates and two recovered from patients. 64.2% out 28 isolates displayed carbapenem resistance, and 66.6% of them were also resistant to betalactams, aminoglycosides and fluoroquinolones. Oxacilinase carbapenemase presence was evident in 17 of 18 isolates resistant to imipenem. Gene bla OXA23 and two betalactamases were detected in isolates resistant to imipenen, with 6.3 and 6.9 isoelectric points. Conclusions. An endemic group of A. baumannii was detected in the 10 month study duration. OXA23 carbapenemase presence was an important factor for carbapenem resistance; however, microbiologic results allow us to assume the possible presence of other resistance mechanisms.

Keywords : Acinetobacter baumannii; carbapenemases; nosocomial infection.

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