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LONDONO, JUAN F; ORTIZ, GLORIA M  and  GAVIRIA, ÁNGELA MARÍA. Prevalence of personnel of the intensive of Medellín 2004. Infect. [online]. 2006, vol.10, n.3, pp.160-166. ISSN 0123-9392.

Objective. Our objective was to determine the prevalence of methicilin resistant Staphylococcus aureus (MRSA) and methicilin sensitive Staphylococcus aureus (MSSA) in the nasal cavity of the personal that labours at the intensive care unit at the Clínica Universitaria Bolivariana. Methodology. In this descriptive study, samples from nasal cavity and pharynx were taken from 45 members of the Intensive Care Unit staff. The samples were seed on plates nurtured with lamb’s blood agar from which we isolated S. aureus colonies that were identified by means of the Gram coloration and the catalase. Coagulase test and manitol fermentation were perfomed in order to confirm S. aureus. The antibiotic sensitivity was determined by the Kirby Bauer method. The oxacillin resistance was confirmed with the Mueller Hinton screening test in oxacillin (6 mg/ml) and NaCl (4%) supplemented media. A diffusion screen test on disk with cefoxitin was performed to MRSA strains to predict whether the methicilin resistance was mediated by the mecA gen. The data was tabulated in Excel and analyzed in SPSS. Results. The prevalence of MRSA was 6.7%. Nasal cavity was the most common anatomical site from which the isolated came

Keywords : Staphylococcus aureus; methicillinresistant; MRSA; oxacillin.

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