Abstract

SANCHEZ, Liliana; RIOS, Rodrigo  and  MATTAR, Salim. Detection of extended spectrum beta-lactamases (ESBL) in Escherichia coli and Klebsiella pneumoniae isolated in a clinic in Villavicencio, Colombia. Infect. [online]. 2008, vol.12, n.3, pp.193-200. ISSN 0123-9392.

Introduction: Multirresistant microorganisms that produce ESBL are one of the main problems in hospitals. Objective: To establish the presence of extended spectrum ß-lactamases (ESBL) in Klebsiella pneumonia and Escherichia coli that produce nosocomial infections in a clinic in Villavicencio, Colombia. Methods: 50 strains were studied, 29 K. pneumoniae and 21 E. coli, from patients with nosocomial infections at a clinic in Villavicencio; CLSI disk diffusion of the test and ESBL MicroScan® as the confirmatory method were used. Results: ESBL producers were determined in 3 of 50 (6%) isolates, 2 of 21 K. pneumoniae and 1 of 29 E. coli. The disk diffusion procedure recommended by CLSI and MicroScan ® ESBL were in agreement about production and confirmation of ESBL (p>0.05). Conclusions: The study showed low ESBL producers in K. pneumoniae and E. coli in a second level clinic in Villavicencio. These results suggest restricting the use of broad spectrum β-lactamases cefalosporins to prevent ESBL dissemination at hospital level.

Keywords : extended spectrum β-lactamases; nosocomial; Villavicencio; Escherichia coli; Klebsiella pneumoniae.

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