SciELO - Scientific Electronic Library Online

 
vol.14 issue1Captive animals at Barranquilla’s zoo are reservoirs of high resistance bacterial pathogensPrevalence of intestinal Protozoa in79 Children 2 to 5 years old from a state nursery in Circasia, Quindío author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Infectio

Print version ISSN 0123-9392

Abstract

SOTO, Juliana et al. Genomic Characterization of HIV-1 in vitro Integration in Peripheral Blood Mononuclear Cells, Macrophages and Jurkat T Cells. Infect. [online]. 2010, vol.14, n.1, pp.20-30. ISSN 0123-9392.

Introduction: Most of the infected host cell genome is available for retroviral integration; however, it has been proposed that this process does not occur at random and depends upon each type of retrovirus. Objective: The objective is to identify and characterize differences in human genome regions of peripheral blood mononuclear cells, macrophages and Jurkat T cells in which integration of HIV-1 occurs. Material and Methods: Three hundred human DNA genome sequences, previously deposited in the GenBank, were selected at random. Using program BLAST, only 264 of them were included in the study because relevant information about chromosomal position, associated genes, repetitive sequences, number of CpG islands and average replication time was available; these sequences were exported to other data bases for analysis. Results: 53% (140/264) of integrations were located on G bands. 70.45% of provirus was located in human genes and the rest was located in repetitive elements. In general the integration site selection was correlated with genomics and structural characteristics of cell chromatin including Alu-Sx and L1 sequences, gene and CpG island densities, remodeling of chromatin, and replication time. All of them would influence the efficient interaction between the pre-integration complex and target cell genomes. Conclusion: It was determined that HIV-1 integration in target cellular genomes would be conditioned by differential characteristics of associated chromatin and by epigenetic processes that would influence the selection of integration sites.

Keywords : Human Immunodeficiency Virus 1; retroviral integration; bioinformatics; human genome.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License