Resumo

DUQUE, Clara M et al. Method Comparison for the Recovery from Streptococcus agalactiae and Its Prevalence Determination in Pregnant Women of Medellín. Infect. [online]. 2010, vol.14, n.2, pp.105-111. ISSN 0123-9392.

Streptococcus agalactiae, is a Gram positive coccus that is frequently associated to infections in neonates, pregnant women, and immunocompromised adults. Objective: Compare method sensitivity for recovery from S. agalactiae and to determine its prevalence in a group of pregnant women of the city of Medellín. Materials and methods: Cross-sectional descriptive study. The population was pregnant women of Medellín who consulted from February to October, 2008; 362 pregnant women were included; the samples were taken from vagina introitus and anal area. The samples were simultaneously cultured in New Granada Agar and Todd Hewitt Broth supplemented with antibiotics, which was the base for the performance of a subculture in Ram Blood Agar at 5%. Results: When comparing the New Granada Agar method of recovery with the Todd Hewitt Broth gold test, the sensitivity of the first was 44% and the positive predictive value was 58%; it presented 98% specificity and a negative predictive value of 99%. S. agalactiae was found in 21 (5.8%) pregnant women. Conclusions: Todd Hewitt Broth is a sensitive method for the recovery from S. agalactiae; therefore, continuing with this methodology for the screening of pregnant women in our environment is recommended. The 5,8% prevalence found in this study is consistent with the reports in world-wide literature. Considering that the rate of vertical transmission is approximately 50%, the need to implement epidemiological surveillance measures in our environment is evident.

Palavras-chave : Streptococcus agalactiae; New Granada Agar; Todd Hewitt Broth; colonization; pregnant women.

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