Abstract

DUARTE, Carolina et al. CLSI microdilution M27-A2 and EUCAST method comparison for Candida spp. in patients with cancer. Infect. [online]. 2010, vol.14, suppl.2, pp.s107-s115. ISSN 0123-9392.

Objective: compare the broth microdilution testing reference standards CLSI M27-A2 and EUCAST, identifying the usefulness of each one of them and their main differences, against the antifungal agents amphotericin B ⁽¹⁾, fluconazole (FCZ), and itraconazole (ITZ) using clinical isolates of Candidaspp. in cancer patients. Methods: isolates of C. albicans (n=136), C. tropicalis (n=36), and Candidaspp. (n=17) were tested by the two methods. The Kappa index was used to establish the degree of agreement between the methods. Results: the degree of agreement between the two methods was high for AB (κ: 1) and FCZ (κ: 0.74) and was low for ITZ (κ: 0.49). Agreement was variable and specific for the various species: for ITZ and FCZ in C. albicans, it was 0.45 and 0.64, respectively. In C. tropicalis, it was of 0.48 and 0.91, and in Candidaspp., it was 0.73 and 0.87 respectively. Discussion: this study suggests that antifungal susceptibility testing using both methods is equivalent. Attention should be focused on differences and discrepancies associated with the species tested, the type of antifungal agent, and the incubation times, which can cause variations at the moment of interpreting the results obtained.

Keywords : Antifungal Agents [pharmacology]; Candida [drug effects]; Candida [isolation & purification]; Candidiasis [microbiology]; Humans; Microbial Sensitivity Tests [methods]; Fluconazole; Amphotericin B; Itraconazole.

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