Abstract

SALVATIERRA, Karina  and  FLOREZ, Hector. Analysis of hepatitis C virus in hemodialysis patients . Infect. [online]. 2016, vol.20, n.3, pp.130-137. ISSN 0123-9392.  https://doi.org/10.1016/j.infect.2015.10.002.

Introduction: Patients with chronic renal failure on hemodialysis comprise a risk group for infection with hepatitis C. This viral infection is the most common cause of chronic liver disease in hemodialysis. Objective: To evaluate the prevalence and to characterise hepatitis C virus infection in a groupof patients from the city Posadas (Argentina) who are on hemodialysis. Materials and methods: The presence of the virus of hepatitis C was evaluated by enzymeimmuno assay of 113 samples of whole blood obtained from patients with chronic renal failure under going hemodialysis. In positive cases, the presence of the viral genome was confirmed byRT-nested PCR of the 5´-noncoding region of the hepatitis C virus, and the viral genotype and subtype were determined by restricted fragment length polymorphism analysis. Results: Of a population of 113 patients with chronic renal failure on hemodialysis, the detection of hepatitis C virus infection was performed by enzyme immunoassay, of which 89.4%(n = 101) of the cases were non-reactive, and 10.6% (n = 12) of cases were reactive. We confirmed the reactive results in 12 cases by RT-PCR, and we determined that these cases were genotype 1a (7/12) and genotype 2a (5/12). Conclusion: The prevalence of infection with hepatitis C virus in this group of patients onhemodialysis was 10.6%, and the hepatitis C virus genotypes found (1a and 2a) were the same as the prevalent genotypes in the infected population of Argentina.

Keywords : Hepatitis C virus; Renal failure; Hemodialysis; Genotypes; Subtypes.

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