Resumo

GARCES-PARADA, Tatiana  e  ARBELAEZ-RAMIREZ, Luis Fernando. Characterization of the non-capsided 3d protein, of the foot-and-mouth disease virus and the production of polyclonal antibodies. Infect. [online]. 2019, vol.23, n.4, pp.376-381. ISSN 0123-9392.  https://doi.org/10.22354/in.v23i4.814.

The noncapsid proteins of the foot and mouth disease are used as markers in the evaluation of animals that have been in contact with the virus, to discriminated the immunized animals, because the vaccine should not have these proteins, therefore animals should not present antibodies against them. The aim of this investigation was the characterization of the 3D non-capsid protein and the production of polyclonal antibodies in vivo. The protein was purified from the culture of inactivated virus, by ion exchange chromatography. The elution of the peaks were submit an one-two-dimensional electrophoresis; Demonstrated a high degree of purity (> 90%) in peak three, where the 3D protein was identified, by the MALDI-TOF technique and ion trap electrospray. The purified protein, inoculated in goats and the hyperimmune serum, was precipitate out and submitted to affinity chromatography to obtain immunoglobulins; the immune reaction was confirmed by means of immunodiffusion and Western blot. The purification process proved to be efficient and useful for obtaining specific antibodies, which will be useful in the preparation of an immunoenzymatic assay that measures the purity of the vaccine against to the content of these proteins.

Palavras-chave : Foot-and-mouth disease virus; Electrophoresis; non-capsid protein; antibodies.

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