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Infectio

Print version ISSN 0123-9392

Abstract

CARVAJAL-VALENCIA, Silvia K.; LIZARAZO, Diana; DUARTE, Carolina  and  ESCANDON, Patricia. Identification of Candida auris isolates recovered through laboratory surveillance in Colombia: a challenge for diagnostic. Infect. [online]. 2020, vol.24, n.4, pp.224-228. ISSN 0123-9392.  https://doi.org/10.22354/in.v24i4.880.

Objective:

To compare the identification results obtained by different identification systems of C. auris isolates.

Methods:

A descriptive study with data collected during the years 2016-19 through surveillance. The results generated by the MicroScan, Phoenix BD, VITEK 2 and MALDI-TOF MS systems of 843 clinical isolates of C. auris submitted to the INS were evaluated and compared with the results generated from confirmation through MALDI-TOF MS (Bruker Daltonics) or PCR.

Results:

Out of 843 clinical isolates submitted to the INS, 81.7% were confirmed as C. auris by MALDITOF MS or PCR in the INS and the rest, 18.3%, were identified as other species of Candida spp. The correct identifications sent by the laboratories was 42.4%. MicroScan identified C. auris as C. haemulonii, C. guilliermondii, C. albicans and C. famata; Phoenix BD, VITEK 2 and MALDI-TOF MS identified C. auris as C. haemulonii.

Discussion:

Studies indicate that C. auris exhibits a close phylogenetic relationship with C. haemulonii. In addition, discrepant identifications may occur because the databases of diagnostic systems are limited with reference to this pathogen. The deficiencies of commercial systems for the identification of C. auris must be complemented with other systems such as MALDI-TOF MS or molecular tests.

Keywords : Candida auris; surveillance; diagnosis; invasive fungal infection.

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