SciELO - Scientific Electronic Library Online

 
vol.9 número4Cáncer de Piel: Perfil Epidemiológico de un Centro de Referencia en Colombia 2003-2005La Metasíntesis: una Metodología de Investigación índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • En proceso de indezaciónCitado por Google
  • No hay articulos similaresSimilares en SciELO
  • En proceso de indezaciónSimilares en Google

Compartir


Revista de Salud Pública

versión impresa ISSN 0124-0064

Resumen

FARFAN, Ana E.; GUTIERREZ, Reinaldo  y  ANGULO, Víctor M.. Using ELISA for identifying Triatominae feeding behaviour in Colombia. Rev. salud pública [online]. 2007, vol.9, n.4, pp.602-608. ISSN 0124-0064.

Objective Standardising the ELISA technique for identifying triatomine insects' feeding behaviour. Methods The ELISA test was standardised by preparing 12 animal anti-specie polyclonal antisera by immunising rabbits with sera from domestic and wild animals; the sera were titred and absorbed to improve specificity. The intestinal content of fifth-instar Rhodnius prolixus (previously fed on each host) was used as positive control; negative controls were obtained from triatomines without feeding. The intestinal content from 60 intradomicile R. prolixus collected in the field was processed to determine the test's effectiveness. Results The high-reactivity ELISA technique was standardised in detecting every blood protein in the positive controls used here. Blood proteins from one or more domestic and wild hosts were detected in 70% of the intestinal content of triatomines collected in homes. Bird, human, pig and goat blood were the most frequent feeding sources; blood proteins from wild animals were identified in 9.5% of them. Conclusions The technique was shown to be effective in detecting blood proteins from different hosts in the intestinal content of triatomines taken from the laboratory and the field. Even though domestic animals' blood was preferentially determined, the findings from wild animals' blood could indicate insect mobility probably from the wild to the domicile. This tool helps in understanding triatomines' behaviour regarding their hosts, thereby contributing to understanding Chagas' disease eco-epidemiology.

Palabras clave : Triatominae; ELISA; feeding behaviour.

        · resumen en Español     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons