Resumo

FARFAN-GARCIA, Ana E.; CASTELLANOS-DOMINGUEZ, Yeny Z.; LUNA-MARIN, Katherine P.  e  ANGULO-SILVA, Víctor M.. Concordance of two serological tests for the diagnosis of Chagas disease. Rev. salud pública [online]. 2013, vol.15, n.2, pp.192-202. ISSN 0124-0064.

Objetive Establish the concordance between in-house ELISA and IIF for the diagnosis of infection with T. cruzi using blood eluates. Methodology A study of diagnostic technology evaluation and cross-sectional sample of 650 residents of an endemic area of Colombia was conducted. It was determined the Receiver Operating Characteristic curve (ROC) and IIF was used as a gold standard. It was established the cutoff for the ELISA and the correlation between readings. Results The in-house ELISA it was an agreement of 0.99 (95 % CI: 0.989 to 0.992) between the two readings taken and the area for the ROC curve was 0.9795. The cutoff was set at 0.5 absorbance in the ELISA test. 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions The in-house ELISA showed good concordance compared to the IIF, so it is a good choice diagnostic for the population living in remote areas.

Palavras-chave : Chagas disease; Trypanosoma cruzi; diagnosis; serology.

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