Abstract

MATULEVICH-PELAEZ, Javier Andrés; CASTRILLON-CARDONA, William Fernando  and  CHITIVA-CHITIVA, Luis Carlos. Phytochemical study and evaluation of antioxidant capacity of leaves of Senna reticulata obtained in the Colombian Andean region. Rev. Cient. [online]. 2017, n.29, pp.149-163. ISSN 0124-2253.  https://doi.org/10.14483/udistrital.jour.rc.2016.29.a4.

The purpose of this study was to contribute to the phytochemical study of its leaves and assess to evaluate its antioxidant capacity. For this purpose, from plant material collected in the municipality of Honda (Tolima) and identified in the Colombian National Herbarium collection under the number COL 573012, the ethanol extract (131.18 g) was obtained from which a mixture consisting of erythritol, isoamyl acetate and L-pyroglutamic acid was obtained and an isolation of quercetin, kaempferol and hyperoside, through separations by column chromatographies (CC), thin layer chromatography (TLC) and thin layer preparative chromatography (TLPC). Quercetin, kaempferol and hyperoside were, for this species first reported in our country. The structural elucidation of the isolated compounds was carried out using techniques of gas chromatography-mass spectrometry (GC-MS) and nuclear magnetic resonance of carbon (13C NMR). The antioxidant capacity was evaluated by the method of DPPH, where it was established that the ethanolic extract, fractions of dichloromethane and ethyl acetate and the compound identified as kaempferol, exhibited a percentage of inhibition higher than 40 %, taking the antioxidant capture of the free radical DPPH gallic acid as reference.

Keywords : DPPH; Kaempferol; phytochemistry; medicinal plants.

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