Abstract

ANDRADE SILVA, Alejandra; MONTENEGRO RUIZ, Luis Carlos; DUQUE ESCOBAR, Santiago Roberto  and  CANO ARANGO, John Edward. First record of Limnothrix vacuolifera and Limnothrix planktonica (Cyanobacteria) in the Colombian Amazon and their antimicrobial activity. Biota colombiana [online]. 2024, vol.25, e1171.  Epub Aug 23, 2024. ISSN 0124-5376.  https://doi.org/10.21068/2539200x.1171.

In recent years Cyanobacteria have become an important source of secondary metabolites. The present work collected, isolated, identified, and characterized the antimicrobial potential of two filamentous species of cyanobacteria from the Colombian Amazon. Microbial techniques streaking in solid medium and dilution in liquid medium were used to obtain strains with a single species. Identification was performed by morphological characters and sequencing the conserved Cya section of the 16s gene. The presence of genes sequencing polyketide synthases (PKS) and non-ribosomal peptide synthetases (NRPS) associated with secondary metabolite-producing pathways were identified by PCR. The antimicrobial activity of polar and non-polar extracts was evaluated with one Gram-positive and two Gram-negative bacteria and a fungal strain. Strain 5 was identified as Limnothrix vacuolifera (Skuja) Komárek and strain 9 as Lymnothix planktonic (Wołoszyńska) Meffert. In both strains PKS and NRPS were found. Finally polar extracts inhibited the growth of Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae and one strain Penicillium sp. Non-polar extracts didn’t show antimicrobial activity. This is the first record of these for the Colombian Amazon, and the first record of antibiotic activity of Colombian continental cyanobacteria.

Keywords : antibiotic; antifungal; Colombian Amazon; cyanobacteria; secondary metabolites.

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