Abstract

VALDERRAMA FONSECA, Ana Milena; ARANGO ISAZA, Rafael  and  AFANADOR KAFURI, Lucia. PLANT TRANSFORMATION MEDIATED BY AGROBACTERIUM: "APPLIED NATURAL GENETIC ENGINEERING". Rev. Fac. Nac. Agron. Medellín [online]. 2005, vol.58, n.1, pp.2569-2585. ISSN 0304-2847.

Agrobacterium tumefaciens has the ability to transfer DNA between different kingdoms. This finding has had a great impact in several fields of plant biology, agriculture and biotechnology.  This review describe the mechanisms by which Agrobacterium  transfer DNA to the plant,  emphasizing 7 fundamental steps in the A. tumefaciens-plant interaction, to provide an opportunity for professionals in the biological sciences to familiarize themselves with the topic.  Basic knowledge about the DNA transfer mechanism has allowed the development of various vectors for the introduction of foreign genes, among which two classes that are currently used are described: co-integrative and binary vectors. Furthermore, detail some important factors that mediate transformation and some of the principal applications of the transformation of plants and fungi by means of Agrobacterium.

Keywords : Genetic transformation; Agrobacterium tumefaciens; genetic engineering; plant biotechnology.

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