Abstract

MUNOZ CRUZ, Walter et al. ESTIMATION OF OPERATION VARIABLES OF A BIOREACTOR WITH Azadirachta indica A. Juss. CELLS. Rev. Fac. Nac. Agron. Medellín [online]. 2006, vol.59, n.2, pp.3467-3478. ISSN 0304-2847.

Operation variables of a stirred tank bioreactor were studied in order to culture cell suspension of Azadirachta indica A. Juss Carboximethylcelulose, CMC 0,7 % w/v, was used to estimate the coefficient of oxygen transfer, kLa, between 120 - 400 rpm and 0,05 - 0,6 vvm, obtaining values of 0,5 - 8,0 h^-1. The kLa for suspension cultures of A. indica in erlenmeyers was 0,6 - 1,2 h^-1. Based upon the previous results, the operation conditions of the bioreactor were defined and cell growth of A. indica was evaluated at 200 rpm and 0,2 vvm of air, reaching 9,2 g dry cell/l. Celular growth was not limited by dissolved oxygen. The sizes of cell agglomerates magnetically stirred in erlemeyers with bafles and in the bioreactor were similar, but smaller that those obtained in erlenmeyers with orbital agitation. The present study establishes parameters for operation of bioreactors with A. indica and confirms that CMC can be used to estimate operational variables in bioreactors.

Keywords : bioreactors; Azadirachta indica; plant cell culture; kLa.

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