Abstract

GARCIA CEPERO, Ana María; LOPEZ FORERO, Yamel  and  RIANO HERRERA, Nestor Miguel. APPLICATION OF A TECHNIQUE OF MOLECULAR EXCLUSION CHROMATOGRAPHY  FOR THE PURIFICATION OF DNA FROM Coffea sp. PLANTS. Rev. Fac. Nac. Agron. Medellín [online]. 2006, vol.59, n.2, pp.3499-3507. ISSN 0304-2847.

One of the greatest difficulties in extracting and purifying biomolecules from plants in the genus Coffea is the high polyphenol and tannin contents. In this study a methodology is described that allows obtaining high purity DNA from leaf tissues of seven genotypes of Coffea sp. by means of the technique desribed by Chaparro (1993) and its further purification was achieved  by molecular exclusion chromatography on Sephacryl S-1000 (Pharmacia). The results showed that the high separation efficiency of degraded RNA, proteins, pigments, and other compounds that absorb between 220 and 300 nm allowed obtaining high purity DNA as judged by the spectophometric and electroforetic data.

Keywords : Coffea arabica  L.; coffee; DNA; molecular exclusion chromatography.

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