Revista Facultad Nacional de Agronomía, Medellín
versão impressa ISSN 0304-2847
REALPE ORTIZ, Carmen Eliana; VILLEGAS GARCIA, Clemencia e RIANO HERRERA, Néstor Miguel. ISOLATION AND MORPHOLOGICAL CHARACTERIZATION OF Rosellinia pepo Pat. IN MACADAMIA PLANTS. Rev.Fac.Nal.Agr.Medellín [online]. 2006, vol.59, n.2, pp. 3509-3526. ISSN 0304-2847.
The fungus Rosellinia pepo Pat, the causal agent of star gall, is considered to be a main phytosanitary problem to the Macademia tree by causing the death of the tree while in its productive stage. Because no reliable isolation method exists that assures recovery of the mushroom with a minimum percentage of contamination and studies of this pathogen are scarce. This study was conducted to perfect an isolation methodology and permit some morphological characterizations of this pathogen. The new methodology allowed isolations with 91,26% purity of mushroom to be obtained. The rate of growth was 4,68 mm day-1. The colonies were of white color and cottony appearance in the beginning, but as the micelio aged it assumed a brown or black color and a brittle appearance. The observation of white star-shaped micelio in the interior side of the synthetic medium allowed differentiation of it from other species like R. bunodes. The microscopic measurements of the swollen peryform yielded 106,4μm longitude on average and 75,3 μm width. This work also allowed determination of the level of infective inocule.
Palavras-chave : Rosellinia pepo; star gall; macadamia; isolation; characterization.