Abstract

CAPATAZ TAFUR, Jacqueline; OROZCO SANCHEZ, Fernando; VERGARA RUIZ, Rodrigo  and  HOYOS SANCHEZ, Rodrigo. ANTIFEEDANT EFFECT OF CELL SUSPENSION EXTRACTS OF Azadirachta indica ON Spodoptera frugiperda J. E. Smith UNDER LABORATORY CONDITIONS. Rev. Fac. Nac. Agron. Medellín [online]. 2007, vol.60, n.1, pp.3703-3715. ISSN 0304-2847.

Extracts of neem (Azadirachta indica) and their active ingredients as azadirachtin, present an action and inhibition of the development of many insects. In this study, the antifeedant effect of cell suspension extracts elicited to different conditions of light and temperature was examined. The extracts were applied in corn leaves disks and submitted to bioassays in larvae of second instar of Spodoptera frugiperda J. E. Smith (Lepidoptera: Noctuidae). The intracellular extracts and the culture media (supernatant) of the cell suspensions of A. indica showed a biological effect on larvae of S. frugiperda L2, with values of the 100 % of antifeeding index for the intracellular extracts of cells elicited to 15 °C and darkness, and 39,3% of antifeeding index for extracellular extracts (medium of cultivation) of elicited suspensions to 35°C , and darkness.

Keywords : Azadirachta indica; azadirachtin; light; temperature; Spodoptera frugiperda.

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