Abstract

CARDONA BUSTOS, Nadya Lorena; BETANCUR PEREZ, Jhon Fredy; RIVERA SERNA, Luis Fernando  and  GAITAN BUSTAMANTE, Alvaro. IDENTIFICATION OF PATHOGENIC CANDIDATES GENES IN THE INTERACTION OF THE CENICAFE 9501 STRAIN WITH THE ROOT KNOT NEMATODE Meloidogyne spp.. Rev. Fac. Nac. Agron. Medellín [online]. 2008, vol.61, n.2, pp.4527-4541. ISSN 0304-2847.

In 1997 it was reported for the first time from soil isolation of a Hyphomycete fungus from Chinchiná ( Caldas, Colombia ), attacking eggs and other biological stages of the plant parasite nematodes Meloidogyne incognita and M. javanica, from commercial coffee plots. Due to the impossibility to classify it taxonomically under current genera, this isolate has been labeled in the mean time as CENICAFE 9501. Given its potential as biological control agent, the identification of canditate genes involved in pathogenicity processes on Meloidogyne eggs was proposed. With this purpose, differential libraries were constructed using the subtractive hybridization method. Sequencing of 188 clones allowed the identification of 80 unigenes, with the highest percentage corresponding to sequences without homology (32%), followed by candidate genes for pathogenesis (22%), cellular transport (17%), protein synthesis (11%) and in lesser degree those involved with transcription and primary metabolism (18%). Among those genes with reading frames showing homology to proteins involved in pathogenicity can be found a peptidase, a ubiquitination receptor, a deubiquitinase, a ubiquinone oxydoreductase, a protein related to the degradation of the cell wall, a glycosyl hydrolase and fatty acid hydrolase, as well as a serine protease. A validation of the putative function of these genes is neccesary in order to increase the basic knowledge of the physiology of this fungus with bioregulation potential.

Keywords : Nematophagous fungi; fungi biocontrol genes; pathogenicity genes; serín proteases; fungi: Meloidogyne Interactions.

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