Abstract

OSORIO-FAJARDO, Armando  and  CANAL, Nelson A. Selection of Strains of Entomopathogenic Fungi for Management of Anastrepha obliqua (Macquart, 1835) (Diptera: Tephritidae) in Colombia. Rev. Fac. Nac. Agron. Medellín [online]. 2011, vol.64, n.2, pp.6129-6139. ISSN 0304-2847.

Fifteen strains from entomopathogenic Beauveria bassiana and Metarhizium anisopliae fungi were evaluated on one day-old adults of Anastrepha obliqua fruit fly. Tested were carried out for selecting the most virulent strains and the effectiveness of their use on young adult when the entomopathogen were applied before emergence were studied too. A screening with a 1x10⁷ conidia/mL concentration was used for selecting the three most pathogenic isolates, two from Beauveria and one from Metarhizium, having 77, 71 and 66% mortality. The LC50 for these isolates were 2.38x10⁶, 1.81x10⁶ and 9.94x10⁶ conidia/mL, respectively, and a respective LT50 were 48.12, 56 and 42.75 hours. No significant differences were found between females and males mortality. LC90 spraying of selected strains on fly pupation medium led to 34-48% mortality at 120 hours. Entomopathogenic fungi could be used for A. obliqua biological control and also it can be used easily spraying towards young adults under tree canopies in the integrated pest management programs.

Keywords : Beauveria bassiana; biological control; fruit flies; Metarhizium anisopliae.

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