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Revista Facultad Nacional de Agronomía Medellín

Print version ISSN 0304-2847

Abstract

ARAMENDIZ TATIS, Hermes; CARDONA AYALA, Carlos  and  LUGO TORRES, Elis Angélica. In Vitro POLLEN GERMINATION OF EGGPLANT (Solanum melongena L.). Rev. Fac. Nac. Agron. Medellín [online]. 2012, vol.65, n.2, pp.6637-6643. ISSN 0304-2847.

Abstract: We assessed the viability of pollen of eggplant (Solanum melongena L.) using in vitro germination method. The collection of flower buds in pre-anthesis of the Lila criolla variety was carried out in the morning from 07:00 to 08:00 hours. Pollen grains were extracted with an electric vibrator and rehydrated in a humid chamber for two hours at a temperature of 25 ºC. Subsequently, for germination in vitro, were dispersed, using a paintbrush, in a culture medium container 100 g of saccharose (C12H22O11), 500 mg of calcium nitrate [Ca (NO3)2 4H2O], 120 mg of magnesium sulfate (MgSO4), 100 mg of potassium nitrate (KNO3) and 120 mg boric acid (H3BO3) dissolved in 1,000 mL of distilled water. Subsequently, it was added 10 g of agar and the pH was adjusted to 6.0. The fresh pollen was incubated in a culture medium for eight hours and observations were made every two hours. The results indicate that the method used is reliable to quantify the viability of pollen grains, because after eight hours of incubation achieved the highest percentage of germination (79%), the pollen tube length was of 0.50 mm and diameter of 0.0532 mm. Therefore, the use of pollen with 8 hours of storage is favorable for hybrid seed production through artificial hybridization, to have been an increase of 0.4942% germination with quadratic effect, for each hour of incubation.

Keywords : Hybrid seed; artificial hybridization; viability of pollen; seed physiology.

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