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Revista Facultad Nacional de Agronomía Medellín

Print version ISSN 0304-2847

Abstract

POSADA CESPEDES, Susana; MATURANA MENA, Diana Milena  and  LOPEZ ORTIZ, Juan Bautista. KARYOTYPE OF TITÍ GREY (Saguinus leucopus) THROUGH R-REPLICATIVE BANDS. Rev. Fac. Nac. Agron. Medellín [online]. 2012, vol.65, n.2, pp.6729-6737. ISSN 0304-2847.

Abstract. Colombia is considered a mega-biodiverse country, boasting several endemic species such as Saguinus leucopus. The Marmoset gray, as is commonly called, inhabits tropical forests, is omnivorous and diurnal, and stands as seed dispersers. Morphologically characterized by small size, brown fur and silver back. It is organized in family groups formed by the couple and their offspring, with a dominant female, which is the only breeding. Due mainly to anthropogenic factors, is listed as endangered species by IUCN and is registered in Appendix I of CITES. Although studies have been conducted on the basic biology of S. leucopus, there are few reports on the cytogenetic evaluation and none on banded karyotype R - Replicative. In this research was performed the karyotype and ideogram with replicating R-bands by incorporating 5'-bromo-2'-deoxyuridine (BrdU) in peripheral blood stimulated with phytohemaglutinine of S. leucopus. The results showed a karyotype 2n = 46, with a fundamental number (NF) of 76. Chromosomes are organized into five groups according to their shape and size. Group A, is made up of three pairs of large submetacentric chromosomes; group B for 5 pairs of chromosomes metacentric or submetacentric average size; group C for 6 acrocentric pairs; and group D by 8 pairs subtelocentric and sexual pair XX/XY. The "X" chromosome is medium submetacentric, and the "Y" is metacentric and the smallest genome. Finally, we propose an R-banded ideogram based Replicative mitosis stage III replication.

Keywords : Tamarin; idiogram; fundamental number; polymorphism.

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