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Revista Facultad Nacional de Agronomía Medellín

Print version ISSN 0304-2847

Abstract

CASTELLANOS ROZO, José et al. Characterization of Carbofuran Degrading Bacteria Obtained from Potato Cultivated Soils with Different Pesticide Application Records. Rev. Fac. Nac. Agron. Medellín [online]. 2013, vol.66, n.1, pp.6899-6908. ISSN 0304-2847.

Eighty-two bacterial isolates with potential Carbofuran degradation activity (Furadan®3SC) were obtained from soils cultivated with the potato variety Unica (Solanum tuberosum) in Silos, Norte de Santander (Colombia), with different records of pesticide application. The bacteria were selected for their ability to grow at 25 °C for 72 h in media containing 200 mg L-1 of analytical Carbofuran as the sole source of carbon and/ or nitrogen. The results showed that ten isolates, 12% of those obtained, grew in the culture media. Eight of theses isolates were obtained from soils with a high pesticide exposure (eight years of application), and identified, by macroscopic, microscopic and biochemical characteristics, as Sphingomonas paucimobilis. The other two were obtained from soils with three years and one year of application and were identified as Pseudomonas aeruginosa and Yersinia pseudotuberculosis, respectively. Subsequently, these bacteria were tested for their ability of hydrolytic degradation of Carbofuran; the results show that the pesticide was degraded only by the isolates of Sphingomonas paucimobilis for 72 h. The results obtained in the in vitro tests show the bacterial metabolic capacity for the biodegradation of Carbofuran, highlighting the potential use of the bacteria for future field evaluation tests in places where residues of the pesticide may exist, as an alternative to control the impact that N-methyl carbamate pesticides have on the environment and human health.

Keywords : Pesticides; Sphingomonas paucimobilis; selection; biodegradation.

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