Resumo

GALLO GARCIA, Yuliana; GUTIERREZ SANCHEZ, Pablo A.  e  MARIN MONTOYA, Mauricio. Detection of PMTV Using Polyclonal Antibodies Raised Against a Capsid-Specific Peptide Antigen. Rev. Fac. Nac. Agron. Medellín [online]. 2013, vol.66, n.2, pp.6999-7008. ISSN 0304-2847.

Potato mop-top virus (PMTV; genus Pomovirus; family Virgaviridae) is the causing agent of the spraing disease in potato (Solanum tuberosum). PMTV is transmitted by Spongospora subterranea f. sp. subterranea (Sss). This disease has a widespread distribution in potato growing regions around the world. The possibility of obtaining strain specific antibodies at low cost can greatly increase the sensitivity and use of serological tests in seed certification programs, plant breeding and quarantine regulations to avoid dissemination of this injurious virus. This work presents an alternative procedure for the production of PMTV specific antibodies useful in serological test such as ELISA and lateral flow. In contrast to standard methods requiring the isolation of viral particles or expression of recombinant capsid, this method uses peptides mimicking the N-terminal region of PMTV capsid protein as antigen for the production of specific polyclonal antibodies. The antibodies were tested against bait plants grown in soil infested with viruliferous Sss, as well as potato plants obtained from naturally Sss infested fields in Colombia. PMTV was detected in 9/14 and 24/28 foliage samples of N. benthamiana and S. phureja, respectively. In the case of field plants, the virus was detected in eight out of 12 root tissues evaluated. The minimum peptide concentration detected by ELISA was of the order of 0.1 nM.

Palavras-chave : ELISA; RT-PCR; Potato mop-top virus; Solanum tuberosum; Spongospora subterranea.

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