Abstract

LOZANO-A., Lucía C.; BAUTISTA, María A.; DUSSAN-G., Jenny  and  VIVES-FLOREZ, Martha J.. DNA EXTRACTION FROM HEAVY OIL CONTAMINATED MICROCOSMS AND RPOB GENE PCR AMPLIFICATION. Actu Biol [online]. 2008, vol.30, n.88, pp.7-14. ISSN 0304-3584.

This work describes a new method for extracting genomic DNA from heavy oil-contaminated soils. This method combines soil washes using three washing solutions with enzymatic lysis (SW-EL method). The suitability of the SW-EL method was validated by extracting DNA from samples as small as 0.5 g from model microcosms comprising soil artificially contaminated with 1 and 10% (w/w) heavy petroleum and seeded with a bacterial consortium. Compared with an established protocol for lysis and DNA purification using the commercial kit GeneReleaserTM (Bioventures) (GRL method), the novel SW-EL method provided higher DNA yields. With the addition of GeneReleaser to the PCR reaction to function as a chelating resin, rpoB genes (serving as model genes) could be successfully amplified. The SW-EL method has potential to be of use for DNA-based analysis of microbial consortia present in heavy-oil contaminated soils.

Keywords : DNA extraction; heavy oil; microcosms; PCR amplification; rpoB.

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