SciELO - Scientific Electronic Library Online

 
vol.30 issue89TOTAL POLYPHENOLS ANALYSIS OF MATURE SEEDS AND TISSUE CULTURES OF SOME COLOMBIAN COCOA VARIETIESSEED SHADOWS, SEEDLING SURVIVAL AND SPATIAL DISTRIBUTION OF THE PALM OENOCARPUS BATAUA, IN A FOREST OF THE COLOMBIAN ANDES author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Actualidades Biológicas

Print version ISSN 0304-3584

Abstract

RIVERA-RODRIGUEZ, Silvia C.  and  DUSSAN-GARZON, jenny. TRIAL EVALUATION OF TOXICITY AND PATHOLOGY IN MURINE TISSUES USING BACILLUS SPHAERICUS COLOMBIAN STRAIN. Actu Biol [online]. 2008, vol.30, n.89, pp.125-134. ISSN 0304-3584.

Colombian isolate Bacillus sphaericus OT4b25 was tested for toxicity and pathology on rabbits and mice as mammalian models. After a high single dose of microorganism (108-109 spores/ml) in four different tests that included dermal, inhalatory, oral, and intraperitoneal routes of administration, subsequent daily observations were performed and weekly body weight was measured. In the oral and intraperitoneal routes infectivity was evaluated by a gross necropsy at interim or final sacrifice, recovering the microorganism both from organs and from peripheral blood. In the oral test, clearance of microorganism was estimated in feces and in the inhalatory test it was estimated from lungs. In both tests a decrease of the recovered microorganism was observed in time. Even though in the oral test recovery of the microorganism from the liver, spleen and lungs and additionally in the intraperitoneal from caecum and injection site was observed, it was not recovered from blood and brain in both tests. Although a persistence of the microorganism in animals was observed after oral, inhalatory and intraperitoneal routes of administration, no animals died or showed lesions during each test, and the body weight was not affected compared with the control groups. In conclusion, at the conditions and doses tested Bacillus sphaericus OT4b25 is not toxic or pathogenic following dermal, oral, inhalatory, and intraperitoneal routes of administration.

Keywords : Bacillus sphaericus; biological control; Colombian isolate; pathology; toxicity.

        · abstract in Spanish     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License