SciELO - Scientific Electronic Library Online

 
vol.33 issue94MICROPROPAGATION AND INDUCTION OF STORAGE ORGANS IN CURCUMA LONGA L.RESPONSE OF THREE GENOTYPES OF TOMATO TO IN VITRO CULTURE AND ISOLATION OF PROTOPLASTS author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Actualidades Biológicas

Print version ISSN 0304-3584

Abstract

CALDERON-ARIAS, Ana M.; RESTREPO-GOMEZ, Andrea  and  URREA-TRUJILLO, Aura I.. MORFOGÉNESIS IN VITRO A PARTIR DE YEMAS APICALES Y BASES DE HOJAS DE LAS ESPECIES DE BROMELIAS AECMEA VEITCHII Y RACINAEA CRISPA. Actu Biol [online]. 2011, vol.33, n.94, pp.17-33. ISSN 0304-3584.

The bromeliads are a promissory resource in our Neotropical forests. However, due to their uncontrolled harvest and the devastation of their habitats, some populations of these plants have declined drastically to the point that many are threatened with extinction. Thus, efficient propagation techniques such as tissue culture constitute an aid to conservation and sustainable management strategies for these resources, as in the case of Aechmea veitchii Baker and Racinaea crispa Baker. To this end, leaf segments of field plants, and apical buds and leaf segments of plantlets cultured in vitro, were used as explants. The effect of different combinations and concentrations of auxin and cytokines on callus formation, type of callus and shoot regeneration were evaluated, using MS medium with half and complete salt concentrations as basal medium. Results showed that there was no response when using explants from field plants; however, in vitro seedlings, adventitious buds were developed on the apical buds of A. veitchii, in MS medium with IBA (1.0 mg/l), TDZ (0.5, 1.0, 1.5 and 2.0 mg/l) with IBA (0.1 mg/l) and BAP (5.0 mg/l) with ANA (0.2 mg/l) induced malformed buds and shoot clusters. A low percentage of adventitious shoots and bud clusters were obtained from the basal region of the young leaves of A. veitchii; however, in MS medium supplemented with kinetin and 2,4D nodular, callus formation was obtained. In vitro propagation of R. crispa from seeds was possible, but its response to the growth regulators evaluated was poor and no plants were regenerated.

Keywords : adventitious shoots; bromeliad; in vitro propagation; organogenesise.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License