Abstract

SALAZAR-MONCADA, Diego A et al. Antagonism of Purpureocillium sp. (UdeA0106 strain) with fungi of flowers cultures. Actu Biol [online]. 2014, vol.36, n.100, pp.22-31. ISSN 0304-3584.

Studies developed in the BIOMA research group of the Universidad de Antioquia showed how the fungus Purpureocillium sp. (strain UdeA0106) may biocontrol a plague known as symphylans (subphylum Myriapoda: class Symphyla). The goal of this study was to identify fungal genera isolated from different substrates (symphylans and soils) and determine their antagonistic capacity, as well a collection of biocontrol strains, over Purpureocillium sp. (strain UdeA0106). Nine fungal strains were isolated from symphylans samples, belonging to 3 genera, and 17 strains were isolated from soil samples, belonging to 10 genera. The antagonism tests consisted of an in vitro test using the dual culture technique in Petri dishes using a sabouraud dextrosa agar (SDA) culture médium, which was conducted on 16 strains: 12 obtained from the isolates and 4 from the collection of the BIOMA research group. The variables evaluated were the growth radius in the confrontations and the percentage of radial growth inhibition (PICR) at 7 and 14 days. Data were analyzed using a generalized linear mixed model using the SAS® Proc GLIMMIX procedure in an independent manner. The fungus Purpureocillium sp. (strain UdeA0106) was inhibited by Volutella sp. (Vo.UdeA0109), Paecilomyces sp. Isolate 2 (Pa.UdeA0109) and Peyronella sp. (Pe.UdeA0108) (p <0,001), while the results of the strains Beauveria sp. (Be.UdeA0109) Dendrodochium sp. (De.UdeA0109), Myrothecium sp. (Mi.UdeA0109), Paecilomyces sp. isolated 1 (Pa.UdeA0309), Paecilomyces sp. isolated 3 (Pa.UdeA0209), Lecanicillium sp. (Le.UdeA0109), and Purpureocillium sp. (Hy.UdeA0109) were not significant. The analyses showed that interactions with undesirable results may occur between fungal strains when conducting control activities.

Keywords : antagonism; biocontrol; isolates; symphylans; Purpureocillium sp.

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